Snodland Town’s record FA Vase run ended with a 3-1 defeat at Reading City in the third round.
Town led through Reggie Rye’s 21st-minute strike but the hosts responded after half-time.
Ellis Cowd levelled and James Clark made it 2-1 from the penalty spot before clinching victory with his second goal in the closing minutes.
But holders Whitstable march on after beating Eastbourne United 3-0 at the Belmont.
Javaun Splatt broke the deadlock after half-time, Nathan Jeche gave the hosts breathing space and Jayden Boulton completed the scoring for Jamie Coyle’s side.
Faversham Strike Force bid to join Whitstable in round four when they host Crawley Down Gatwick, also at the Belmont, on Sunday.
Fisher are also flying the flag for the Southern Counties East league, beating Fleet 8-7 on penalties following a 2-2 draw.
Duane Acheampong scored twice, including a late winner, as Hollands & Blair won a seven-goal Premier Division thriller at Holmesdale.
Kane Rowland and George Goodwin gave Blair an early 2-0 lead but Malachi Hudson’s double ensured the teams went in level at half-time.
Acheampong put the visitors back in front just after the break and settled the match two minutes from time after Callum Keeble had made it 3-3.
Hythe Town dropped into the bottom two after losing 4-0 at home to Phoenix Sports.
Phoenix player-boss Jake Goodman opened the scoring on his return to Reachfields as the visitors led 1-0 at the break.
Jamarie Brissett’s quickfire double and an injury-time Tyrese Agbontaen strike sent Sports clear.
Chislehurst Glebe climb out of the relegation zone at the Cannons’ expense after beating Kennington 5-1 at home.
Khavarn Williams (2), Elliott Wenham, Jamie Philpot and Frankie Sawyer scored for the hosts, while Lucas Mvongo grabbed Ton’s consolation.
Hosts Rusthall were 2-0 up against Corinthian, thanks to goals from Louie Clarke and Charlie Clover, when the match was abandoned due to floodlight failure.
Bearsted’s match at Sutton Athletic was postponed following a pitch inspection.
